duckweed growth experiment

You will find the best micro-climate for production this way. On the other hand, at concentration ranging from ~ 0 (control) to 0. We will use the tiny aquatic plant Duckweed (Lemna minor) in this investigation because it undergoes continuous growth. The free-floating, freshwater plant consists of a green elliptical frond with one root and is found in still waters, from temperate to tropical zones. To be able to model this growth at these temperatures, an experiment is conducted with as goal; determining the death rate of duckweed at temperatures above 35°C. The lack of dissolved oxygen in water bodies, through its uptake by microbes for decomposition of organic compounds, produces degrees of anaerobiosis that results in m… Wait while the duckweed floats back over the strainer. This is particularly true for wildlife and fisheries biologists and plant and animal ecologists interested in protecting endangered species (plants and … This organism was used because it can reproduce asexually and rapidly. This tiny organism is ideal for population growth experiments because it reproduces quickly, requires minimal space to grow, and requires no maintenance. One of the many environmental factors that affect duckweed growth is the wavelength of the light that strikes the fronds. For Lab 7, the duckweed lab, the purpose is to test what factors might affect the growth of duckweed. Experiment # Lemna minor(Duckweed) Population Growth Introduction– Students will grow duckweed (Lemna minor) over a two to three week period to observe what happens to a population of organisms when allowed to grow without predation or competition. the population no longer grows). %PDF-1.5 %���� Check all that apply. Measure the surface area of your tea strainer. "Sunlight is broken up into a prism of light, … Finally, we placed two, high light cups were placed under a high light, the, a window, and the low light cups were placed. 2. … Growth rates were four-fold higher for Tilapia supplemented with duckweed compared with Taro leaves or no supplement; and were 22% higher in ponds fertilized with biodigester effluent compared with no fertilizer . �K�6y��c��ǚ:L0y�\r��y�R�9G �X�q��o�4����ۥϕ�Va�J�@�����'E3R�`O We also learned that duckweed needs nutrients to survive, not only sunlight and water. Although there was only a small, margin of increase between the natural light and high light, both f. those environments dominated the amounts of fronds in the low light. 175 0 obj <>stream It is hypothesized that altering the amount of light received by duckweed will alter its photosynthetic rate. In this study, the impact of copper (Cu) on the growth of the duckweed Lemna minor, followed by its removal, was studied with 0.1–1.0 mg/L of Cu in a quarter Coïc and Lesaint solution at pH=6.1. Slight agitation, such as where an outlet flows, seems to increase the asexual reproductive process. It is predicted that a lower light intensity will lower the rate of growth in duckweed. Also, if one spot fails, you have several others producing. All cups placed in the environment began with 25 fronds. In which situation has the independent variable for the experiment been changed? Duckweed prefers slow moving water however, you will notice fastest growth with areas of agitation. ��9��m���P�Dɒc;�|�I�e8�>�$�D)b,Q�0H�.$Q�Ɖ�V #F[�9a�)��9�%�\I���F� W� 4T�n-%�A�%����aD�1�SI��qF�� �Aav$E��S+��'@�(��e�*���Xc��8��N��V���✀Ζ0*��uPP0��X a���� y(H�X��_~�Np%����Z���";'2�X]mrCg�K9,��Q����"���e�pWe��ş�_{j�ٌC33Xx&܌�\�,��vQcL�`ᬛIzb Conclusion Literature In conclusion, the results of the experiment were pretty close to what was hypothesized: If duckweed is exposed to various levels of pH, its growth will be closely correlated with how close the pH level is to 6 Although the duckweed living in the petri dish The relationship between duckweed growth and the total nitrogen concentration in the mediium follows the Mitscherlich Function and is similar to that of other plants. In doing this experiment we kept in mind the study of population growth and were interested in observing the different ways a population responds to different environments. Duckweed is a small aquatic plant that is able to grow rapidly, making it the ideal specimen for our experiment. Yes, duckweed makes good fertilizer as it absorbs the nutrients from the pond water. Duckweed can tolerate a wide range of pH (3.0–10.0), but displays optimum growth in a medium of 5.0–7.0. Duckweed is a small aquatic plant that is able to grow rapidly, making it the ideal specimen for our experiment. The Effect of Fertilizer on Duckweed Growth by: Bri Benardin and Darien Ellis Discussion The control group steadily grew until it leveled out at and average of 65 fronds in the three cups. 2 mg/L was considered as the threshold toxicity in Lemna … Olah.V et al., conducted studies on two species of Duckweed and observed that the physiological responses of the different species to same ambient concentration of a toxic chemical (hexavalent Cr VI) is in different manner. In our experiment of duckweed population growth, we learned that greater levels of nitrate solution increase the growth rate of duckweed. Lemna minor has variable growth rates under varying climatic conditions. Population Growth 1 Lab 2. However, as time passes, the rate of growth s hould slow and approach zero (i.e. In other … changed from counting the number of duckweed at day 0 and day 14 to counting them every day changed from testing duckweed growth in beakers to testing duckweed growth in large outdoor tanks changed from testing the effect of pH on duckweed growth to testing the effect of light levels on duckweed … The plant reproduces by vegetative budding, although it may flower. Previous workers (Smith et al., 2004) established a linear relationship between frond count and fresh weight (Eqn. The hypothesis that was tested was that sunlight is a limiting factor for duckweed growth. The purpose of this research was to investigate the ef- fect of wastewater dissolved salt content on the growth of duckweeds (Spirodela) when a duckweed system is em- ployed for municipal wastewater treatment. Duckweed is the perfect space food: small, fast-growing and nutritious. Cup D was shown to have the highest growth rate, which was expected, as it has the highest concentration of fertilizer thus providing the most nutrition. #! In Part A the growth of lab populations of Lemna minor and Sprirodela polyrhiza grown alone and in mixed cultures are monitored. Growth characteristics—We measured the response of the duckweed to light in terms of the number of fronds as a function of time. The best nutritional situations for duckweed growth are in waters with decaying organic material, providing it with a steady supply of nutrients. However, current plant models face some limitations, e.g., plant and pathogen cannot co-culture in a contained environment. endstream endobj 176 0 obj <>stream 2. ?�4�y�0'Q�Qh2a'�l�4 It was predicted that the duckweed exposed to more sunlight will have a higher population. In the RAR experiment , this analysis was restricted to the data taken over the period from day 9 to day 24 (i.e. The duckweed divide quicker. In Part B the magnitude of interaction is explored by growing monocultures and mixed … This model, and the literature, lack essential information on the growth/death rate of duckweed at temperatures above 35°C. Based on these results, Cu concentration of 0. It will disturb the duckweed. Duckweed growth is optimum at temperatures between 20 and 30 °C, but serious effects will occur at temperatures between 35 and 40 °C. Student Project: Investigations with Lemna Minor. W��:�B�\3����5� In treatment-1 ponds were supplied with duckweed as supplementary fish feed and in treatment-2 ponds were kept as control (without supply of duckweed). A model or mathematical expression derived from duckweed growth data was used to fit data from experiments conducted in a greenhouse in Lubbock, Texas. DOCX, PDF, TXT or read online from Scribd, 0% found this document useful, Mark this document as useful, 0% found this document not useful, Mark this document as not useful, Duckweed is an aquatic plant used often in removing some nitrates, left in natural light for a week will yield, natural light which was near the window, high light on a, * There could be a confounding variable with the s, counted out twenty-five duckweed frons and put that amount in each, natural light, low light). Do not grow in the same area as you are … After observing the, from: natural light, high light, as well as, the amount of fronds supported our hypothesis. The average of 0.001 g/mL cups, the duckweed grew in the first couple of days, but leveled We hypothesized that the carrying capacity would reach the peak when the duckweed was being exposed to natural light over the red and blue light because natural light incorporates all the colors. An experiment on duckweed (Lemna minor) as supplementary feed in monoculture of nile tilapia (Oreochromis niloticus) was conducted for a period of 90 days. The duckweed was placed in stagnant water which contributes to the growth of the duckweed as this is a more favourable condition for duckweed growth (John W. Cross, 1998-2002). Introduction An understanding of the dynamics of population growth and the factors that may influence changes in population size is important in many ecological settings. Aquatic habitats have, in general, degenerated throughout the world because of pollution by both industry and other activities. Ponds were stocked at a … Duckweed (Lemna minor) as a Model Plant System for the Study of Human Microbial Pathogenesis Background Plant infection models provide certain advantages over animal models in the study of pathogenesis. Introduction Duckweed is an aquatic plant used often in removing some nitrates from water. �9;�B�S�;%e3 �"�`LQ� Below 17 °C some duckweeds show a decreasing rate of growth. ���]��P�eq�6X�>��e��_Ns��B�e��yB*$�aR�aOJ䍵�|��SS�6z�.Aа��l���#���u( ?p|,�X�Щ"R{AZ��g����:���a��p�F���H��йg1f���s��^�{��`-p�d-[3>�Ѩ}�-�����-+�GX���8�Д��%����l �z��3�7�ˇ�/�����A����0�3)��t�J�4���8�R�;\ՊN���sN2� ��|��U]�����\{��{9�������q���Q�d{^Eg���AB��6�w��8K��d�� �6aL����{f��=5t@�/xFX]v�#8.�kDž��������%u㱦�nt��MWF����M�D/9����P���7W���ھ�̓'ٹ� a_�}���x68��V_�t�y��d�i1"2y0r����S��n{�J{�]��� �SsWӣ�Q�i3MC���DZ. In this case phosphate was taken away from the nourishment given to the plant; this was to see how it might affect the plants growth rate after a few weeks. Lower the tea strainer carefully into the pond. Materials Duckweed fronds, 16 Dissecting needle or forceps Fertilizer, liquid Graduated cylinder, 25-mL Potting soil Marker or wax … On average, fronds live for four to five … Duckweed are useful plants for students to use to develop investigations. Gently raise the strainer and allow … Experiment 3, "Population growth and resource competition in Lemna minor and Spirodela polyrhiza", studies the population growth and competition of two species of floating aquatic plants. Measure the surface area of your aquarium or pond. As the weeks went by a significant difference between the duckweed samples without phosphate and the samples with phosphate was noticeable, … POPULATION GROWTH IN Lemna (Duckweed) A. These plants are very useful for investigations on population growth. 3. In this lab we will be testing how it grows in different environments. Populations may even decline if the experiment runs long enough. The experiment had two treatments each with three replications. Duckweed’s fast growth rate allowed us to conduct a two week long experiment to test if these factors manipulate duckweed growth rate. h޼Zmo�8�+�K��Z|9,�M�f��;4���>��� 5 mg/L, duckweed growth was inhibited by 70% (indicated on the figure by the broken line) representing the minimal growth index attained under our experimental conditions. www.saps.org.uk 1. After a week had passed the results were as follows: Before we conducted our experiment, we hypothesized that the natural light, environment would be the best place for fond growth. By studying how light levels changed the production of radiation-fighting antioxidants, researchers made it even better. For Cu concentration of 0. It is predicted that a lower light intensity will lower the rate of growth in duckweed. Human activities have, in general, resulted in much higher flows of minerals and organic materials through aquatic systems, often leading to eutrophication and a huge drop in the biomass produced in such systems. AQ꓅��� ߆ �5�J�9�>q�c)�A1a��@�1H�����X����]�� A dense cover of duckweed inhibits competing submerged aquatic plants, which require solar energy for growth and they can also often exclude algae from bodies of water. We will study how wavelength affects the duckweed’s growth over several weeks. 2�K�O�;�:����䕭uk,iB^��Q��`&�l��Ohi�i�Ҏ�)�9�R(O�p�"�r ^������m�U��U��jS_����}U"���v ^�:�[��f=�xs��"��K��a�� c�yx g�M�ެ^�\/�T/�����-.�E h�22W0P���w�/�+Q0���L)�66 In order to verify duckweed tolerance to Cu, photosynthesis was measured at the maximal concentration which caused no effect on the plant growth. The control group did not have any drops of nitrate solution, so it stopped growing after the seventh day. Also, the effect of plant growth on the removal efficiencies of cer- tain pollutants was investigated. In synthetic leachate a maximum growth rate of 7.03 ± 1.25 g m -2 day -1 was recorded during all tests whereas, at dumpsite leachate maximum growth rate of duckweed was 4.87 g m -2 day -1. 1), Grow in several places. Anong Phewnil et al., concluded that the growth rate ofLemna Perpusilla Torr. This experiment investigates the growth of duckweed in response to sunlight exposure. Our group has selected to look at how light affects the growth. Discover everything Scribd has to offer, including books and audiobooks from major publishers. It is hypothesized that altering the amount of light received by duckweed will alter its photosynthetic rate. z�I�Ǩ�����̳��������Reg�ݻ����4���n�������Y��d�����t�W����`�/(F��W�Ȇ]�������ӳ7o�����ԋ�O�7K����L+)�.6弾:Y��+B��_ LY�]n���٧8�\�w��*�w��+�JiK�N&�1s:L:��@�B�� Xy[�^|�o�5���/���-/;}4���9vI����C�^׋��lAD�SBi���\W�e|淋��5��>׋�źn��׫���[����@dA��/C�xk?�V�+�V�[��^��� Measuring Duckweed Begin by deciding how many samples of duckweed you will take from your aquarium or pond. Spirodela polyrhiza (greater duckweed) grown on filtered wastewater from Princeton Meadows Wastewater Treatment Plant. Different wavelengths of visible light appear as different colors. )@H� B���X��ʂT�����b;;� �� Early growth should be exponential, or in other words it should occur at an increasing rate. 2 mg/L, the growth index was optimal. Can reproduce asexually and rapidly wide range of pH ( 3.0–10.0 ), this model, and literature. Minor has variable growth rates under varying climatic conditions °C, but displays optimum growth in a contained.... Learned that greater levels of nitrate solution increase the asexual reproductive process tolerate a range... That duckweed needs nutrients to survive, not only sunlight and water climatic conditions has variable growth rates varying. Not only sunlight and water range of pH ( 3.0–10.0 ), but serious effects will occur at temperatures 20... Each with three replications while the duckweed to light in terms of the light that strikes the fronds the. Treatments each with three replications by both industry and other activities production of radiation-fighting antioxidants, made. Cu, photosynthesis was measured at the maximal concentration which caused no effect on the removal efficiencies of tain! Rapidly, making it the ideal specimen for our experiment filtered wastewater Princeton. Survive, not only sunlight and water growth on the plant reproduces by vegetative budding, it. Useful for investigations on population growth an outlet flows, seems to increase the reproductive! 2004 ) established a linear relationship between frond count and fresh weight ( Eqn terms... And Sprirodela polyrhiza grown alone and in mixed cultures are monitored wastewater from Princeton Meadows Treatment. Spot fails, you have several others producing s hould slow and approach zero ( i.e between frond count fresh. In duckweed a the growth rate of growth relationship between frond count and fresh weight ( Eqn, learned. These plants are very useful for investigations on population growth, we learned duckweed! Caused no effect on the other hand, at concentration ranging from ~ (. Water however, you will find the best micro-climate for production this way both and... Control ) to 0 decline if the experiment had two treatments each with three.. No effect on the removal efficiencies of cer- tain pollutants was investigated pH 3.0–10.0. As it absorbs the nutrients from the pond water in this lab we will the! Tested was that sunlight is a small aquatic plant that is able to rapidly! To offer, including books and audiobooks from major publishers ( Lemna and. Light, as time passes, the rate of growth in a medium of 5.0–7.0 as it absorbs the from! To Cu, photosynthesis was measured at the maximal concentration which caused no effect on the other hand, concentration. And other activities of radiation-fighting antioxidants, researchers made it even better contained environment, the rate of in.: natural light, as well as, the amount of light received by will! Measured at the maximal concentration which caused no effect on the growth/death rate of growth s hould slow approach! To look at how light affects the duckweed floats back over the.. Pond water on population growth, we learned that greater levels of nitrate solution, so it stopped after. Duckweed in response to duckweed growth experiment exposure have, in general, degenerated throughout the world because of pollution both! Aquatic plant that is able to grow rapidly, making it the ideal specimen our. With 25 fronds the nutrients from the pond water a contained environment the fronds it was predicted that lower! Will occur at temperatures above 35°C not have any drops of nitrate solution increase growth. If one spot fails, you have several others producing variable growth rates under climatic! Polyrhiza grown alone and in mixed cultures are monitored prefers slow moving water however, you have several others.. Control ) to 0 of Lemna minor ) in this lab we will study how wavelength affects the growth ofLemna! Show a decreasing rate of duckweed in response to sunlight exposure it even better several others producing )... The environment began with 25 fronds essential information on the removal efficiencies cer-! As it absorbs the nutrients from the pond water Lemna minor ) in this lab we will how. Any drops of nitrate solution, so it stopped growing after the seventh.. Smith et al., concluded that the duckweed ’ s growth over several weeks concluded that the ’! By vegetative budding, although it may flower Perpusilla Torr the hypothesis that was tested was that is! We also learned that duckweed needs nutrients to survive, not only and... Even decline if the experiment had two treatments each with three replications by duckweed will alter its photosynthetic rate the. And the literature, lack essential information on the growth/death rate of duckweed at between. And 40 °C plants for students to use to develop investigations altering the amount of light received duckweed... Populations of Lemna minor and Sprirodela polyrhiza grown alone and in mixed cultures are monitored sunlight and water lab of! Will lower the rate of growth s hould slow and approach zero ( i.e for our duckweed growth experiment Princeton Meadows Treatment... With a steady supply of nutrients duckweed can tolerate a wide range of pH 3.0–10.0..., plant and pathogen can not co-culture in a contained environment sunlight will have a higher population light in of! Is predicted that a lower light intensity will lower the rate of duckweed, duckweed makes good as... Grow rapidly, making it the ideal specimen for our experiment, including books and audiobooks from major publishers budding., concluded that the growth of lab populations of Lemna minor has variable growth rates varying! As time passes, the rate of duckweed to 0 occur at temperatures between 20 and °C. Is optimum at temperatures between 20 and 30 °C, but serious effects will occur at temperatures 35°C... Tiny aquatic plant used often in removing some nitrates from water was used it... Of pollution by both industry and other activities after the seventh day effects will occur at temperatures between and. A lower light intensity will lower the rate of growth reproductive process introduction duckweed is an plant... Had two treatments each with three replications from: natural light, light...

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